Thursday, 5 April 2018

A One-step Protein Purification & Isolation Method for High Purity, Activity, and Yields

Protein purification is a set of methods that are used for isolating or extracting proteins from a complex mixture such as animal or plant tissues, cells, or whole organism. The process of purification helps separate the mixture’s protein and non-protein parts. Purification and extraction are vital components of any protein-specific research; however, the methods used for this usually depend on the nature of both the solution and protein. Chromatography, ultrafiltration, western blotting, precipitation, and electrophoresis are the key methods or technologies.

Protein purification

Chromatography: A Widely Used Method

The chromatography segment led the protein purification and isolation technology segment in 2016 as it is considered one of the accurate and sensitive methods. The segment is expected to expand even during the forecast period (from 2017 to 2025) with a growth rate of 8.3 %. Many companies also focus on the commercialization of their new products based on chromatography to increase their business.

For example, a new affinity chromatography-based method has been developed at the University of Alabama, which helps enhance high-activity, high-purity, and high-yield purification of complex proteins. According to a professor of biochemistry and molecular genetics at the university, this method offers a number of advantages to both researchers and the pharma industry. The professor also said that this one-step purification process overcomes some major weaknesses of the existing commercially available methods.

The chromatography technology is sub-categorized into size exclusion chromatography, affinity chromatography, ion exchange chromatography, hydrophobic interaction chromatography, and reversed phase chromatography. These sub-categories help users decide the purification method depending on their applications and find applications in primary process for academic research and are likely to witness a steady growth over the next few years.

Market Insights

Based on a research report by Grand View Research, Inc.; protein purification and isolation market was estimated at USD 4.7 billion in 2016 and is likely to expand at a CAGR of 8.1 % from 2017 to 2025. A rise in research in the field of pharma and biotechnology for the development of drugs is considered a key factor driving the market demand. In addition, rising need for purification kits to purify, screen, concentrate, and prepare the samples in shorter period is also expected to fuel the market growth. Sigma-Aldrich Corp.; Thermo Fisher Scientific; Bio-Rad Laboratories, Inc.; Norgen Biotek Corp.; and Promega Corp. are some of the key companies in the global market.

In-Depth Research Report On Protein Purification & Isolation Market:
https://www.grandviewresearch.com/industry-analysis/protein-purification-isolation-market

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